[1]刘青,李永,盛世美.金线莲多糖抗衰老作用及其机制[J].华侨大学学报(自然科学版),2020,41(1):77-83.[doi:10.11830/ISSN.1000-5013.201907059]
　LIU Qing,LI Yong,SHENG Shimei.Anti-Aging Effects and Mechanisms of Anoectochilus roxburghii Polysaccharose[J].Journal of Huaqiao University(Natural Science),2020,41(1):77-83.[doi:10.11830/ISSN.1000-5013.201907059]
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金线莲多糖抗衰老作用及其机制()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第41卷
期数:
2020年第1期
页码:
77-83
栏目:
出版日期:
2020-01-20

文章信息/Info

Title:
Anti-Aging Effects and Mechanisms of Anoectochilus roxburghii Polysaccharose
文章编号:
1000-5013(2020)01-0077-07
作者:
刘青;  李永;  盛世美
华侨大学 化工学院, 福建 厦门 361021
Author(s):
LIU Qing;  LI Yong;  SHENG Shimei
College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
关键词:
金线莲多糖;  NF-κB信号通路;  抗氧化;  认知能力;  抗衰老
Keywords:
Anoectochilus roxburghii polysaccharose;  NF-κB signaling pathway;  antioxidant;  cognitive ability;  anti-aging
分类号:
R285;S567
DOI:
10.11830/ISSN.1000-5013.201907059
文献标志码:
A
摘要:
考察金线莲多糖抗衰老作用及其机制.每天给小鼠皮下注射D-半乳糖溶液(40 mg·kg-1)诱导其衰老,同时灌胃给予金线莲多糖(100,200,300 mg·kg-1)或维生素E(200 mg·kg-1)进行治疗,以维生素E治疗组为阳性对照.6周后对小鼠进行行为学测试,8周后检测小鼠的大脑皮层p-NF-κB p65表达,以及大脑皮层过氧化氢酶、超氧化物歧化酶及血清总抗氧化能力活性和丙二醛浓度,并检测小鼠腹腔巨噬细胞的吞噬情况.结果表明:与衰老小鼠相比较,经金线莲多糖治疗后,小鼠的抗氧化酶活性显著提高,其大脑皮层p-NF-κB p65表达下调,且运动、空间探索和学习记忆能力明显改善;同时,金线莲多糖能增强衰老小鼠清除抗原的能力;金线莲多糖抗衰老作用与其抗氧化、抑制NF-κB信号通路、增强衰老小鼠免疫能力有关.
Abstract:
The anti-aging effect and mechanisms of Anoectochilus roxburghii polysaccharose(ARP)was studied. The aging mouse model was induced by subcutaneous injection of D-galactose solution(40 mg·kg-1)daily, then the treated mice were intragastrically administered with ARP(100, 200 and 300 mg·kg-1)or Vitamin E(200 mg·kg-1), and Vitamin E treatment group was used as a positive control. After 6 weeks, the behavioral tests were performed. All mice were sacrificed after 8 weeks, and the expression of p-NF-κB p65 and antioxidant enzymes including superoxide dismutase and catalase in the cerebral cortex were examined. The activity of total antioxidant enzyme activity and malondialdehyde content in serum were measured and the phagocytic ability of mouse peritoneal macrophages were detected. The results showed that compared with aging mice, treatment with ARP could increase the activity of antioxidant enzymes significantly and reduce p-NF-κB p65 expression in cerebral cortex, and increase the movement abilities, space exploration, learning and memory abilities of aging mice. Meanwhile, ARP could enhance the ability of aging mice to clear antigen. The ARP anti-aging effect was due to its antioxidant effect, the inhibition of NF-κB signaling pathway and the improved immunity.

参考文献/References:

[1] HARMAN D.Aging: A theory based on free radical and radiation chemistry[J].Journal of Gerontology,1956,11(3):298-300.DOI:10.1093/geronj/11.3.298.
[2] 孙玉军,江昌俊,任四海.秀珍菇多糖对D-半乳糖致衰老小鼠的保护作用[J].食品科学,2017,38(5):251-256.DOI:10.7506/spkx1002-6630-201705041.
[3] 武燕,张弘,布仁,等.肉苁蓉多糖对D-半乳糖所致急性衰老模型保护作用研究[J].中国药理学通报,2017,33(7):927-933.DOI:10.3969/j.issn.1001-1978.2017.07.009.
[4] 中国科学院中国植物志编辑委员会.中国植物志: 第17卷[M].北京:科学出版社,2000.
[5] 刘青,刘珍伶,周娟.金线莲多糖的体外抗氧化活性[J].华侨大学学报(自然科学版),2010,31(6):21-23.DOI:10.11830/issn.1000-5013.2010.06.0718.
[6] 许丹妮,李健,马玉芳,等.金线莲多糖对免疫抑制小鼠脾淋巴细胞增殖及免疫器官的影响[J].中兽医医药杂志,2011,30(3):23-27.DOI:10.3969/j.issn.1000-6354.2011.03.004.
[7] 马玉芳,郑小香,郑乃珍,等.金线莲多糖协同ConA对小鼠脾淋巴细胞分泌Th1、Th2型细胞因子及其mRNA表达量的影响[J].中国食品学报,2018,18(4):72-77.DOI:10.16429/j.1009-7848.2018.04.011.
[8] 邓燕群,李伟,张晓辉,等.金线莲多糖对α-葡萄糖苷酶活性及糖尿病小鼠血糖的影响[J].汕头大学学报(自然科学版),2014,29(3):41-45.
[9] 余杰,李伟,张晓辉,等.金线莲多糖抗糖尿病作用的研究[J].海峡药学,2013,25(11):18-21.
[10] 翁秀华,王长连,袁曦,等.金线莲对人前列腺癌PC-3细胞增殖能力的影响[J].中国医院药学杂志,2011,31(13):1083-1087.
[11] LI Le,LI Yumeng,LIU Zhenling,et al.The renal protective effects of Anoectochilus roxburghii polysaccharose on diabetic mice induced by high-fat diet and streptozotocin[J].Journal of Ethnopharmacology,2016,178:58-65.DOI:10.1016/j.jep.2015.12.002.
[12] 虞舟,白冬,张海玲,等.鲣鱼内脏中鱼油对D-半乳糖制衰老模型小鼠抗衰老的影响[J].浙江海洋大学学报(自然科学版),2018,37(2):129-134.
[13] 周岩飞,金凌云,王琼,等.薏苡仁油对小鼠免疫功能影响的研究[J].中国油脂,2018,43(10):77-81.DOI:10.3969/j.issn.1003-7969.2018.10.016.
[14] QU Zhuo,ZHANG Jingze,YANG Honggai,et al.Protective effect of tetrahydropalmatine against D-galactose induced memory impairment in rat[J].Physiology and Behavior,2016,154(11):114-125.DOI:10.1016/j.physbeh.2015.11.016.
[15] ZHA Longying,CHEN Jiading,SUN Suxia,et al.Soyasaponins can blunt inflammation by inhibiting the reactive oxygen species-mediated activation of PI3K/Akt/NF-κB pathway[J].PLoS One,2014,9(9):e107655.DOI:10.1371/journal.pone.0107655.
[16] 马骋,阮清伟,俞卓伟.氧化应激与免疫衰老[J].中华老年医学杂志,2017,36(2):224-228.DOI:10.3760/cma.j.issn.0254-9026.2017.02.025.

相似文献/References:

[1]刘青,刘珍伶,周娟.金线莲多糖的体外抗氧化活性[J].华侨大学学报(自然科学版),2010,31(6):718.[doi:10.11830/ISSN.1000-5013.2010.06.0718]
　LIU Qing,LIU Zhen-ling,ZHOU Juan.Study on Antioxidative Activity of Anoectochilus formosams Polysaccharide in Vitro[J].Journal of Huaqiao University(Natural Science),2010,31(1):718.[doi:10.11830/ISSN.1000-5013.2010.06.0718]

备注/Memo

备注/Memo:
收稿日期: 2019-07-22
通信作者: 刘青(1970-),女,副教授,博士,主要从事天然药物的提取及活性的研究.E-mail:yunliu@126.com.
基金项目: 福建省自然科学基金资助项目(2013J01337)
更新日期/Last Update: 2020-01-20