[1]江伟,肖昕,王昆,等.费氏弧菌检测土壤联合毒性模型分析[J].华侨大学学报(自然科学版),2021,42(6):809-816.[doi:10.11830/ISSN.1000-5013.202101028]
 JIANG Wei,XIAO Xin,WANG Kun,et al.Combined Toxicity Model Analysis of Soil Detection With Vibrio fischeri[J].Journal of Huaqiao University(Natural Science),2021,42(6):809-816.[doi:10.11830/ISSN.1000-5013.202101028]
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费氏弧菌检测土壤联合毒性模型分析()
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《华侨大学学报(自然科学版)》[ISSN:1000-5013/CN:35-1079/N]

卷:
第42卷
期数:
2021年第6期
页码:
809-816
栏目:
出版日期:
2021-11-12

文章信息/Info

Title:
Combined Toxicity Model Analysis of Soil Detection With Vibrio fischeri
文章编号:
1000-5013(2021)06-0809-08
作者:
江伟12 肖昕2 王昆2 吴晋芝2 张良2 陆方筱2
1. 华侨大学 福建省生物化工技术重点实验室, 福建 厦门 361021;2. 华侨大学 化工学院, 福建 厦门 361021
Author(s):
JIANG Wei12 XIAO Xin2 WANG Kun2WU Jinzhi2 ZHANG Liang2 LU Fangxiao2
1. Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, Xiamen 361021, China; 2. College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
关键词:
土壤污染 费氏弧菌 重金属离子 毒性检测 模型分析 响应面分析法
Keywords:
soil pollution Vibrio fischeri heavy metal ions toxicity detection model analysis response surface analysis
分类号:
X530.2
DOI:
10.11830/ISSN.1000-5013.202101028
文献标志码:
A
摘要:
在获得费氏弧菌(Vibrio fischer)相对发光强度与重金属离子浓度关系的基础上,采用响应面分析法建立相对发光强度与土壤联合毒性的数学模型.同时,对五处正在从事生产的工厂土壤进行采样检测验证模型的可行性.结果表明:通过建立的模型成功得出五处采样地点的土壤状况.其中,五处采样点中浅层土生物毒性均大于深层土,同一工厂排污口和生产车间之间土壤生物毒性差异较小;各采样点土样中的离子联合毒性均位于模型中的Ⅱ级毒性区间,相当于重金属离子含量为0.01 mg·L-1.说明,该模型可用于初步判断土壤生物毒性.
Abstract:
Based on the relationship between the relative luminous intensity of Vibrio fischeri and the concentration of heavy metal ions, a response surface analysis method was used to establish a mathematical model between the relative luminous intensity and the soil combined toxicity, and the feasibility of the model was verified by sampling and testing of the soil from five factories that were engaged in production The result shows that the soil conditions at five sampling sites are successfully obtained through the model. The biological toxicity of top soil is greater than that of deep soil, and the difference between the sewage outlet and the production workshop of the same factory is small. The combined toxicity of the ions in the soil samples at each sampling point is located in Grade Ⅱ toxicity interval in the model, which is equivalent to a heavy metal ion content of 0.01 mg·L-1. This means the model can be used for the preliminary judgment of soil biological toxicity.

参考文献/References:

[1] 曾鸣,林志芬,尹大强,等.混合污染物联合毒性研究进展[J].环境科学与技术,2009,32(2):80-86.DOI:10.3969/j.issn.1003-6504.2009.02.021.
[2] 石昊,袁孟杰,孟一鸣,等.铜、铬和敌敌畏对费氏弧菌的联合毒性效应研究[J].环境与发展,2019,31(3):105,107.DOI:10.16647/j.cnki.cn15-1369/X.2019.03.061.
[3] MA Xiaoyan,WANG Xiaochang C,NGO H H,et al.Bioassay based luminescent bacteria: Interferences, improvements, and applications[J].Science of the Total Environment,2014,468/469:1-11.DOI:10.1016/j.scitotenv.2013.08.028.
[4] LEON M B,ALBRECHT J A.Comparison of adenosine triphosphate(ATP)bioluminescence and aerobic plate counts(APC)on plastic cutting boards[J].Journal of Foodservice,2007,18(4):145-152.DOI:10.1111/j.1745-4506.2007.00060.x.
[5] 朱丽娜.基于发光细菌法的水质综合毒性研究[D].北京:中央民族大学,2012.DOI:10.7666/d.y2137416.
[6] ABBAS M,ADIL M,EHTISHAM-UL-HAQUE S,et al.Vibrio fischeri bioluminescence inhibition assay for ecotoxicity assessment: A review[J].Science of the Total Environment,2018,626:1295-1309.DOI:10.1016/j.scitotenv.2018.01.066.
[7] 杨虹.常见重金属对费氏弧菌的生物毒性研究[J].环境科学与管理,2015,40(10):140-142.DOI:10.3969/j.issn.1673-1212.2015.10.033.
[8] 赵莉,杨虹,郭晶晶.二元含汞重金属混合物对费氏弧菌的联合毒性研究[J].安全与环境学报,2015,15(1):297-300.DOI:10.13637/j.issn.1009-6094.2015.01.062.
[9] 王雨潇,任慧平,皇甫益.镍氢电池在电动汽车上的发展[J].包钢科技,2019,45(1):95-98.DOI:10.13647/j.cnki.btgkj.2019.01.024.
[10] CARELLI G,LAVICOLI I.Defining hormesis: The necessary tool to clarify experimentally the low dose-response relationship[J].Human and Experimental Toxicology,2002,21(2):103-104,113-114.DOI:10.1191/0960327102ht 219oa.
[11] SHEN Kaili,SHEN Chaofeng,LU Yuan,et al.Hormesis response of marine and freshwater luminescent bacteria tometal exposure[J].Biological Research,2009,42(2):183-187.DOI:10.4067/S0716-97602009000200006.
[12] JIAN Qijie,GONG Liang,LI Taotao,et al.Rapid assessment of the toxicity of fungal compounds using luminescent Vibrio qinghaiensis sp. Q67[J].Toxins,2017,9(10):335.DOI:10.3390/toxins9100335.
[13] ZOU Xiaoming,LIN Zhifen,DENG Ziqing,et al.Novel approach to predicting hormetic effects of antibiotic mixtures on Vibrio fischeri[J].Chemosphere,2013,90(7):2070-2076.DOI:10.1016/j.chemosphere.2012.09.042.
[14] 汤淼,曾鸿鹄,王大力,等.四环素对费氏弧菌产生生毒物兴奋效应(Hormesis)的时间关系和机制[J].环境化学,2015,34(11):1981-1987.DOI:10.7524/j.issn.0254-6108.2015.11.2015051403.
[15] 蒋媛媛,孟芹,苏嘉缘,等.明亮发光杆菌连续培养条件的优化[J].华东理工大学学报(自然科学版),2016,42(1):48-53,109.DOI:10.14135/j.cnki.1006-3080.2016.01.008.
[16] 陈水松,王英才,胡文,等.不同条件对2种发光菌毒性测试的影响研究[J].环境科学与技术,2013,36(增刊2):1-5,36.
[17] BAVELLONI A,PIAZZI M,RAFFINI M,et al.Prohibitin 2: At a communications crossroads[J].IUBMB Life,2015,67(4):239-254.DOI:10.1002/iub.1366.

备注/Memo

备注/Memo:
收稿日期: 2021-01-20
通信作者: 江伟(1987-),男,副教授,博士,主要从事合成生物学与生物信息学、生物催化与生物转化,以及应用酶学与定向进化的研究.E-mail:wjiang@hqu.edu.cn.
基金项目: 福建省泉州市科技计划项目(2018C008); 华侨大学高层次人才科研启动项目(600005-Z17Y0072)
更新日期/Last Update: 2021-11-20