[1]王镇发,陈培钦,邓秩韬,等.反胶束萃取阿魏酸酯酶[J].华侨大学学报(自然科学版),2013,34(2):182-185.[doi:10.11830/ISSN.1000-5013.2013.02.0182]
　WANG Zhen-fa,CHEN Pei-qin,DENG Yi-tao,et al.Purification of Feruloyl Esterase Using Reverse Micelles Extraction[J].Journal of Huaqiao University(Natural Science),2013,34(2):182-185.[doi:10.11830/ISSN.1000-5013.2013.02.0182]
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反胶束萃取阿魏酸酯酶()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第34卷
期数:
2013年第2期
页码:
182-185
栏目:
出版日期:
2013-03-20

文章信息/Info

Title:
Purification of Feruloyl Esterase Using Reverse Micelles Extraction
文章编号:
1000-5013(2013)02-0182-04
作者:
王镇发;  陈培钦;  邓秩韬;  谢晨;  李夏兰
华侨大学 化工学院, 福建 厦门 361021
Author(s):
WANG Zhen-fa;  CHEN Pei-qin;  DENG Yi-tao;  XIE Chen;  LI Xia-lan
College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
关键词:
阿魏酸酯酶;  反胶束;  萃取;  十六烷基三甲基溴化铵
Keywords:
feruloyl esterase;  reverse micelles;  extraction;  cetrimonium bromide
分类号:
Q556.1
DOI:
10.11830/ISSN.1000-5013.2013.02.0182
文献标志码:
A
摘要:
利用十六烷基三甲基溴化铵(CTAB)/正己醇/异辛烷反胶束萃取阿魏酸酯酶.考察pH值、离子强度、CTAB浓度对阿魏酸酯酶的CTAB/正己醇/异辛烷反胶束萃取及其反萃取的影响.结果表明:萃取的最佳条件为酶液透析24 h,pH值为12,萃取液为25 mmol·L-1 CTAB的正己醇/异辛烷溶液(体积比为1∶5),萃取率接近100%;反萃取的最佳条件为pH值为7的0.20 mol·L-1 KCl溶液,反萃取率可达79%.纯化后比活为356.7 μkat·g-1,纯化倍数为6.9,SDS-PAGE电泳显示两条带.
Abstract:
The cetrimonium bromide/hexanol/isooctane reverse micelles extraction and backward extraction were used to purify feruloyl esterase. The effects of pH, ionic strength and cetrimonium bromide(CTAB)concentration on the cetrimonium bromide/hexanol/isooctane reverse micelles extraction and backward extraction were studied. The results showed that the extraction efficiency was nearly 100% when the optimal extraction conditions were used as followed: enzyme dialysis time 24 h, pH 12 and CTAB concentration in hexanol/isooctane solution(volume ratio 1∶5)25 mmol·L-1. The optimal condition of backward extraction was 0.20 mol·L-1 KCl with pH 7, and the efficiency of backward extraction was 79%. After extraction and backward extraction of the enzyme, specific activity was 356.7 μkat·g-1, purification fold was 6.9, and SDS-PAGE electrophoresis showed two bands.

参考文献/References:

[1] CREPIN V,FAULDS C,CONNERTON I.Functional classification of the microbial feruloyl esterases[J].Applied Microbiology and Biotechnology,2004,63(6):647-652.
[2] MACKENZIE C R,BILOUS D,SCHNEIDER H,et al.Induction of cellulolytic and xylanolytic enzyme systems in Streptomyces spp.[J].Applied and Environmenta Microbiology,1987,53(12):2835-2839.
[3] FAULDS C B,WILLIAMSON G.The purification and characterization of 4-hydroxy-3-methoxycinnamic(ferulic)acid esterase from Streptomyces olivochromogenes[J].Microbiology,1991,137(10):2339-2345.
[4] TOPAKAS E,VAFIADI C,CHRISTAKOPOULOS P.Microbial production, characterization and applications of feruloyl esterases[J].Process Biochemistry,2007,42(4):497-509.
[5] BENOIT I,DANCHIN E G J,BLEICHRODT R J,et al.Biotechnological applications and potential of fungal feruloyl esterases based on prevalence, classification and biochemical diversity[J].Biotechnology Letters,2008,30(3):387-396.
[6] KOSEKI T,FUSHINOBU S,SHIRAKAWA H,et al.Occurrence, properties, and applications of feruloyl esterases[J].Applied Microbiology and Biotechnology,2009,84(5):803-810.
[7] FAZARY A E,JU Y H.Feruloyl esterases as biotechnological tools: Current and future perspectives[J].Acta Biochimica et Biophysica Sinica,2007,39(11):811-828.
[8] 李夏兰,胡雪松,范韵敏,等.一株产阿魏酸酯酶青霉菌株的筛选、鉴定及生长特征[J].微生物学通报,2010,37(11):1588-1593.
[9] 李夏兰,范韵敏,方柏山.来自桔青霉的阿魏酸酯酶的分离纯化、理化性质[J].微生物学报,2010,50(8):1058-1064.
[10] 汪家正,范明.蛋白质技术手册[M].北京:科学出版社,2000:42-47.
[11] 王金枝,曹学君.反胶束萃取技术分离胰激肽原酶[J].中国生物工程杂志,2007,27(3):93-99.
[12] KOSEKI T,FUSHINOBU S,SHIRAKAWA H,et al.Occurrence, properties and applications of feruloyl esterases[J].Applied Microbiology and Biotechnology,2009,84(5):803-810.
[13] 李凯,李成付,李加友,等.反胶束萃取精氨酸脱亚胺酶[J].精细化工,2008,25(2):163-166.

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备注/Memo

备注/Memo:
收稿日期: 2012-04-18
通信作者: 李夏兰(1965-),女,教授,主要从事生物化工的研究.E-mail:xialan@hqu.edu.cn.
基金项目: 福建省科技计划重点基金资助项目(2011N0020); 华侨大学科研基金资助项目(09X0289); 华侨大学高层次人才启动基金资助项目(11BS221)
更新日期/Last Update: 2013-03-20